{"id":1965,"date":"2023-10-24T17:15:18","date_gmt":"2023-10-24T15:15:18","guid":{"rendered":"https:\/\/genocan.eu\/?p=1965"},"modified":"2023-11-29T13:13:33","modified_gmt":"2023-11-29T12:13:33","slug":"comparison-of-isag-2006-vs-isag-2020-dog-dna-profiles","status":"publish","type":"post","link":"https:\/\/genocan.eu\/cs\/news\/comparison-of-isag-2006-vs-isag-2020-dog-dna-profiles\/","title":{"rendered":"Srovn\u00e1n\u00ed DNA profil\u016f u ps\u016f ISAG 2006 vs. ISAG 2020"},"content":{"rendered":"

DNA profily u ps\u016f pat\u0159\u00ed mezi z\u00e1kladn\u00ed n\u00e1stroje pro identifikaci plemene, ov\u011b\u0159en\u00ed p\u016fvodu a pochopen\u00ed genetick\u00fdch marker\u016f. Mezi hlavn\u00ed aplikace a vlastnosti genetick\u00e9ho profilov\u00e1n\u00ed DNA pat\u0159\u00ed:<\/p>\n\n\n\n

\n
\n

1. jednozna\u010dn\u011b identifikovat psa<\/strong><\/p>\n\n\n\n

2. nem\u011bn\u00ed se v pr\u016fb\u011bhu \u017eivota<\/strong><\/p>\n\n\n\n

3. lze identifikovat i zv\u00ed\u0159e bez nebo nefunk\u010dn\u00edho mikro\u010dipu<\/strong><\/p>\n\n\n\n

4. ov\u011b\u0159en\u00ed rodi\u010dovstv\u00ed (paternity, maternity)<\/strong><\/p>\n\n\n\n

5. ov\u011b\u0159en\u00ed \u201eclear\u201c po rodi\u010d\u00edch<\/strong><\/p>\n<\/div>\n<\/div><\/div>\n\n\n\n

<\/p>\n\n\n\n

Pro testov\u00e1n\u00ed DNA profil\u016f se vyu\u017e\u00edvaj\u00ed dv\u011b metodiky: ISAG 2006, kter\u00fd vyu\u017e\u00edv\u00e1 kr\u00e1tk\u00e9 tandemov\u00e9 repetice (STR), a nov\u011bj\u0161\u00ed ISAG 2020, kter\u00fd vyu\u017e\u00edv\u00e1 jednonukleotidov\u00e9 polymorfismy (SNP). Tento \u010dl\u00e1nek p\u0159in\u00e1\u0161\u00ed stru\u010dn\u00e9 srovn\u00e1n\u00ed obou metod a objas\u0148uje, pro\u010d je st\u00e1le v\u00edce up\u0159ednost\u0148ov\u00e1na nov\u011bj\u0161\u00ed metoda ISAG 2020.<\/p>\n\n\n\n

ISAG 2006: Profilov\u00e1n\u00ed na z\u00e1klad\u011b STR<\/strong><\/p>\n\n\n\n

Metoda ISAG 2006 vyu\u017e\u00edv\u00e1 p\u0159ibli\u017en\u011b 21 STR marker\u016f. STR neboli kr\u00e1tk\u00e9 tandemov\u00e9 repetice jsou kr\u00e1tk\u00e9 sekvence DNA, kter\u00e9 se po sob\u011b n\u011bkolikr\u00e1t opakuj\u00ed. U ps\u016f je tuto metodu mo\u017en\u00e9 pou\u017e\u00edt nap\u0159. pro:<\/p>\n\n\n\n

    \n
  1. Identifikace zv\u00ed\u0159at<\/strong>: DNA profil slou\u017e\u00ed jako jedine\u010dn\u00fd otisk DNA ka\u017ed\u00e9ho zv\u00ed\u0159ete.<\/li>\n\n\n\n
  2. Ov\u011b\u0159en\u00ed rodi\u010dovstv\u00ed<\/strong>: STR jsou vysoce polymorfn\u00ed, co\u017e umo\u017e\u0148uje potvrdit otcovstv\u00ed nebo mate\u0159stv\u00ed.<\/li>\n<\/ol>\n\n\n\n

    Limitace metody ISAG 2006:<\/span><\/p>\n\n\n\n

      \n
    1. Omezen\u00e1 genetick\u00e1 informace<\/strong>: S pouh\u00fdmi 21 STR markery je prostor pro komplexn\u00ed genetickou anal\u00fdzu omezen\u00fd.<\/li>\n\n\n\n
    2. Omezen\u00ed ov\u011b\u0159en\u00ed rodi\u010dovstv\u00ed<\/strong>: V n\u011bkter\u00fdch p\u0159\u00edpadech je k ur\u010den\u00ed otcovstv\u00ed vy\u017eadov\u00e1n tak\u00e9 profil DNA matky.<\/li>\n<\/ol>\n\n\n\n

      ISAG 2020: Profilov\u00e1n\u00ed na z\u00e1klad\u011b SNP<\/strong><\/p>\n\n\n\n

      Metoda ISAG 2020 zahrnuje hodnocen\u00ed p\u0159ibli\u017en\u011b 220 marker\u016f SNP. SNP neboli jednonukleotidov\u00e9 polymorfismy jsou varianty v DNA na \u00farovni jednotliv\u00fdch DNA baz\u00ed v DNA. Pro genetick\u00e9 profilov\u00e1n\u00ed nab\u00edzej\u00ed \u0159adu v\u00fdhod:<\/p>\n\n\n\n

        \n
      1. Vy\u0161\u0161\u00ed rozli\u0161en\u00ed<\/strong>: Vzhledem k v\u011bt\u0161\u00edmu po\u010dtu marker\u016f poskytuj\u00ed SNP podrobn\u011bj\u0161\u00ed genetick\u00fd profil.<\/li>\n\n\n\n
      2. Stabiln\u011bj\u0161\u00ed<\/strong>: SNP jsou obecn\u011b stabiln\u011bj\u0161\u00ed ne\u017e STR markery, co\u017e vede k men\u0161\u00edmu po\u010dtu mutac\u00ed, a tedy ke konzistentn\u011bj\u0161\u00edm v\u00fdsledk\u016fm.<\/li>\n\n\n\n
      3. \u0160irok\u00e9 pou\u017eit\u00ed<\/strong>: SNP lze pou\u017e\u00edt k identifikaci rodi\u010dovstv\u00ed, plemene, ov\u011b\u0159en\u00ed sourozenc\u016f, odhadu heterozygotnosti.<\/li>\n<\/ol>\n\n\n\n

        V\u00fdhody metody ISAG 2020:<\/span><\/p>\n\n\n\n

        <\/p>\n\n\n\n

          \n
        1. Komplexn\u00ed anal\u00fdza<\/strong>: D\u00edky desetkr\u00e1t v\u011bt\u0161\u00edmu po\u010dtu marker\u016f nab\u00edz\u00ed ISAG 2020 \u0161ir\u0161\u00ed genetick\u00fd p\u0159ehled.<\/li>\n\n\n\n
        2. P\u0159esn\u011bj\u0161\u00ed<\/strong>: Stabilita SNP zaji\u0161\u0165uje vy\u0161\u0161\u00ed m\u00edru p\u0159esnosti.<\/li>\n\n\n\n
        3. Budoucnost<\/strong>: S rozvojem oboru genomiky je d\u00edky hust\u00e9 \u0161k\u00e1le SNP marker\u016f zaji\u0161t\u011bna relevance metody pro r\u016fzn\u00e9 aplikace.<\/li>\n\n\n\n
        4. Pokro\u010dilej\u0161\u00ed funkce anal\u00fdzy<\/strong>: ISAG 2020 umo\u017e\u0148uje posouzen\u00ed heterozygotnosti, potvrzen\u00ed sourozenc\u016f a m\u016f\u017ee umo\u017enit potvrzen\u00ed dal\u0161\u00edch p\u0159\u00edbuzensk\u00fdch vztah\u016f (nap\u0159. babi\u010dka – vnuk).<\/li>\n<\/ol>\n\n\n\n
          Anal\u00fdza<\/strong><\/td>ISAG 2006<\/strong><\/td>ISAG 2020<\/strong><\/td><\/tr>
          Genetick\u00e1 identita jedince<\/td>\u2714<\/td>\u2714\u2714<\/td><\/tr>
          Ov\u011b\u0159en\u00ed maternity<\/td>\u2714<\/td>\u2714<\/td><\/tr>
          Ov\u011b\u0159en\u00ed paternity<\/td>\u2714<\/td>\u2714<\/td><\/tr>
          Heterozygotnost<\/td>\u2718<\/td> \u2714<\/td><\/tr>
          Ov\u011b\u0159en\u00ed sourozenectv\u00ed<\/td>\u2718<\/td> \u2714<\/td><\/tr>
          Heterozygotnost ve vrhu<\/td>\u2718<\/td> \u2714<\/td><\/tr>
          Popula\u010dn\u00ed genetika, diverzita plemene<\/td>\u2718<\/td>\u2714<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

          Zat\u00edmco metoda ISAG 2006 dob\u0159e slou\u017eila komunit\u011b chovatel\u016f a \u00fa\u010del\u016fm genetick\u00e9ho testov\u00e1n\u00ed ps\u016f, metoda ISAG 2020 se je komplexn\u011bj\u0161\u00ed a perspektivn\u011bj\u0161\u00ed. Rozsah dat, kter\u00e9 poskytuje 220 marker\u016f SNP, spolu s jejich stabilitou a \u0161irokou pou\u017eitelnost\u00ed \u010din\u00ed metodu ISAG 2020 vhodn\u011bj\u0161\u00ed pro ty, kte\u0159\u00ed hledaj\u00ed co nejp\u0159esn\u011bj\u0161\u00ed a nejhlub\u0161\u00ed genetick\u00e9 poznatky o sv\u00fdch psech.<\/p>\n\n\n\n

          O\u010dek\u00e1v\u00e1 se, \u017ee metoda ISAG 2020 se v bl\u00edzk\u00e9 budoucnosti stane standardem pro DNA profilov\u00e1n\u00ed ps\u016f.<\/p>\n\n\n\n

          Proto\u017ee metody ISAG 2006 a 2020 nejsou kompatibiln\u00ed, nemohou chovatel\u00e9 porovn\u00e1vat form\u00e1ty ISAG 2006 a 2020.<\/p>\n\n\n\n

          Z v\u00fd\u0161e uveden\u00fdch d\u016fvod\u016f se chovatel\u016fm doporu\u010duje pou\u017e\u00edvat nov\u011bj\u0161\u00ed DNA profil dle ISAG 2020.<\/strong><\/p>","protected":false},"excerpt":{"rendered":"

          DNA profily u ps\u016f pat\u0159\u00ed mezi z\u00e1kladn\u00ed n\u00e1stroje pro identifikaci plemene, ov\u011b\u0159en\u00ed p\u016fvodu a pochopen\u00ed genetick\u00fdch marker\u016f. Mezi hlavn\u00ed aplikace a vlastnosti genetick\u00e9ho profilov\u00e1n\u00ed DNA pat\u0159\u00ed: 1. jednozna\u010dn\u011b identifikovat psa 2. nem\u011bn\u00ed se v pr\u016fb\u011bhu \u017eivota 3. lze identifikovat i zv\u00ed\u0159e bez nebo nefunk\u010dn\u00edho mikro\u010dipu 4. ov\u011b\u0159en\u00ed rodi\u010dovstv\u00ed (paternity, maternity) 5. ov\u011b\u0159en\u00ed \u201eclear\u201c po rodi\u010d\u00edch […]<\/p>\n","protected":false},"author":2,"featured_media":1995,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[17],"tags":[],"class_list":["post-1965","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/posts\/1965","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/comments?post=1965"}],"version-history":[{"count":0,"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/posts\/1965\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/media\/1995"}],"wp:attachment":[{"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/media?parent=1965"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/categories?post=1965"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/genocan.eu\/cs\/wp-json\/wp\/v2\/tags?post=1965"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}